Recovery of hibernating myocardium using stem cell patch with coronary bypass surgery

ObjectiveThis study aims to investigate the utility of mesenchymal stem cells (MSCs) applied as an epicardial patch during coronary artery bypass graft (CABG) target hibernating myocardium; that is, tissue with persistently decreased myocardial function, in a large animal model.MethodsHibernating myocardium was induced juvenile swine (n = 12) using surgically placed constrictor on left anterior descending artery, causing stenosis without infarction. After 12 weeks, single-vessel CABG performed internal thoracic graft. During CABG, region consisting either MSCs grown onto polyglactin mesh 6), or sham 6). Four weeks after and placement, cardiac magnetic resonance imaging examined by gross inspection, including dilators for vessel patency, electron microscopy, protein assays, proteomic analysis.ResultsCABG + MSC showed improvement contractile function (78.24% ± 19.6%) compared (39.17% 5.57%) inotropic stimulation (P < .05). Compared control, microscopy mitochondrial size, number, morphology; analysis similarly increases expression biogenesis marker peroxisome proliferator-activated receptor gamma coactivator 1-alpha (0.0022 0.0009 vs 0.023 0.009) .01) along key components transport chain, succinate dehydrogenase (complex II) (0.06 0.02 0.14 0.03) .05) adenosine triphosphate synthase V) (2.7 0.4 4.2 0.26) .05).ConclusionsIn myocardium, placement cell shows promise improving morphology function.